One‐ and two‐dimensional 90.5‐MHz 13C‐NMR spectroscopy of the N‐linked triantennary oligosaccharide units of calf fetuin
- 18 August 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 204 (2) , 257-260
- https://doi.org/10.1016/0014-5793(86)80823-7
Abstract
Complete assignments of all anomeric resonances in the proton and carbon spectra of the N‐linked oligosaccharide units of fetuin were made using one‐ and two‐dimensional NMR spectroscopy. We are able to confirm the presence of microheterogeneity in the N‐acetylneuraminic acid linkages to the galactose residues and the presence of a unique triantennary structure which carries a side chain: NeuAcα(1–3)Galβ(1–3) GlcNacβ(1–4)‐. Anomeric carbon chemical shifts changes resulting from long‐range conformational effects were observed.Keywords
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