Mass Spectrometric Analysis of Cytokinins in Plant Tissues
Open Access
- 1 July 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 72 (3) , 858-863
- https://doi.org/10.1104/pp.72.3.858
Abstract
The cytokinin complex of Datura innoxia Mill. crown gall tissue was purified by ion exchange, Sephadex LH-20 chromatography and reversed-phase high performance liquid chromatography. By gas chromatography-mass spectrometry using 2H-labeled compounds, the following cytokinins were identified in the basic fraction eluting from a cation exchange column: zeatin, zeatin riboside, dihydrozeatin, dihydrozeatin riboside, their corresponding O-glucosides, 7- and 9-glucosides of zeatin, 9-glucoside of dihydrozeatin, isopentenyladenine, and isopentenyladenosine. Zeatin riboside 5′-monophosphate was the major cytokinin nucleotide in the tissue. In addition, dihydrozeatin riboside and isopentenyladenosine were identified in the nucleotide fraction following enymic degradation.This publication has 16 references indexed in Scilit:
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