Mutagenesis of the catalytic subunit of rabbit muscle protein phosphatase-1
- 1 November 1993
- journal article
- Published by Springer Nature in Molecular and Cellular Biochemistry
- Vol. 127-128 (1) , 113-119
- https://doi.org/10.1007/bf01076762
Abstract
We have generated site-directed mutants of the catalytic subunit of rabbit muscle ppase-1. Since it is known that ppase-1 and ppase-2A are highly susceptible to inactivation by sulfhydryl reagents, we have mutagenized the six cysteine residues conserved between these two enzymes to serines. The six mutants were purified to near homogeneity by affinity chromatography on inhibitor-2-Sepharose and characterized. All six exhibited enzymatic activity. These results indicate that the catalytic mechanism of ppase-1 is different from that of the protein tyrosine phosphatases which involve a cysteinyl phosphate intermediate.Keywords
This publication has 52 references indexed in Scilit:
- Polymerase chain reactions using Saccharomyces, Drosophila and human DNA predict a large family of protein serine/threonine phosphatasesFEBS Letters, 1992
- Identification of Members of the Protein Phosphatase 1 Gene Family in the Rat and Enhanced Expression of Protein Phosphatase 1α Gene in Rat Hepatocellular CarcinomasJapanese Journal of Cancer Research, 1990
- Protein serine/threonine phosphatases; an expanding familyFEBS Letters, 1990
- Sequence homology between purple acid phosphatases and phosphoprotein phosphatasesFEBS Letters, 1990
- Regulation of protein phosphatase‐1G from rabbit skeletal muscleEuropean Journal of Biochemistry, 1989
- Two isoforms of protein phosphatase 1 may be produced from the same geneFEBS Letters, 1988
- A simple and rapid method for the selection of oligodeoxynucleotide-directed mutantsGene, 1988
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- A protein inhibitor of rabbit liver phosphorylase phosphataseBiochemical and Biophysical Research Communications, 1975
- Activation of phosphorylase phosphatase by a novel procedure: Evidence for a regulatory mechanism involving the release of a catalytic subunit from enzyme-inhibitor complex(es) of higher molecular weightBiochemical and Biophysical Research Communications, 1974