GENERATION OF AN INTERLEUKIN-I-LIKE LYMPHOCYTE-STIMULATING FACTOR AT INFLAMMATORY SITES - CORRELATION WITH THE INFILTRATION OF POLYMORPHONUCLEAR LEUKOCYTES

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  • 1 January 1984
    • journal article
    • research article
    • Vol. 65  (5) , 521-532
Abstract
Using a mouse thymocyte co-stimulation assay, thymocyte-stimulating activity was demonstrated in murine peritoneal fluid obtained from the early stage (3-9 h) of casein-induced inflammation. This early inflammatory stage coincided with the time at which an influx of polymorphonuclear leukocytes (PMN) into the inflamed sites was observed. Similar thymocyte-stimulating activity was produced in vitro by PMN purified from 4 h peritoneal exudate but not by purified PMN obtained at a later stage (24 h) of the inflammation. The inflammatory factor was interleukin (IL)-I-like; it was devoid of IL-2 activity when tested with IL-2-dependent cells. It could stimulate murine thymocytes to produce IL-2. On a Sephadex G-75 column, the factor was eluted between the molecular sizes of 10,000 and 30,000; its peak activity was at 21,000. The factor mainly consisted of 2 (pI [isoelectric point] 6.5 and pI 5.0) iso-electrophoretically different factors.