The effects of the hypocholesteremic compound 3β‐(β‐dimethylaminoethoxy)‐androst‐5‐en‐17‐one on the sterol and steryl ester composition ofSaccharomyces cerevisiae

Abstract
When yeast was grown in the presence of 10−4 M 3β-(β-dimethylaminoethoxy)-androst-5-en-17-one (DMAE-DHA), the compound 2,3;22,23-dioxidosqualene (DOS) accumulated. Total free sterol was reduced by about 30%, whereas almost no steryl esters were found. The same drug at lower concentration (3×10−6 M) caused a slight increase in steryl ester production, and a 24% reduction in free sterol content. The marked accumulation of ergostra-5,7,22,24(28)-tetraen-3β-ol with 3×10−6 M DMAE-DHA indicated that the C24–28 reductase is especially sensitive to the action of the drug.