Repression of the Alkaline Phosphatase of Vibrio cholerae

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Abstract
The synthesis of alkaline phosphatase by 2 strains of V. cholerae belonging to the Inaba and Ogawa serotypes was examined in relation to the phosphate concentration of the culture medium. The synthesis of the enzyme in both strains was repressed in cells grown in the presence of a high concentration of Pi. Lowering the phosphate content of the growth medium led to a depression of enzyme activity. The presence of glucose in low phosphate medium stimulated the degree of derepression. The synthesis of the enzyme by strain Inaba 569B was more sensitive to Pi than that of strain Ogawa 154. The enzyme was presumably located in the periplasmic space since it was released when the organisms was converted to spheroplasts. [The alkaline phosphatase of these 2 strains of V. cholerae was studied to determine whether this enzyme could be used as an indicator of serotype or as a marker of toxicity.].