A Method for Separating Small Quantities of MSH and ACTH with Good Recovery of Each

Publisher Website
Google Scholar
Abstract
A method has been developed for separating small quantities of MSH (melanocyte stimulating hormone) from ACTH (adrenocorticotropic hormone) with good recovery of each hormone. The method is suitable for separation of these hormones in acetic acid extracts of pituitary tissue, acetic acid extracts of tumor tissue, or desalted extracts of human plasma. The procedure entails the application of the extract to a semimicro column of SE-Sephadex, from which MSH is eluted with aqueous solutions of pyridine and acetic acid of pH 5-7 and ACTH is displaced with ammoniacal solutions of pH 8-11. The method has been used to demonstrate that there occurs in the plasma and tissues of patients with certain clinical disorders a melanocyte-stimulating substance that is distinct from ACTH.

This publication has 16 references indexed in Scilit: