IgE heavy chain constant region genes in atopic dermatitis and senile erythroderma patients

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Abstract
By Southern hybridization using a genomic DNA fragment carrying a human IgE heavy chain constant region gene (C ε ) as a probe, we analyzed the organization of human C ε genes and their flanking regions in 23 atopic dermatitis and 6 senile erythroderma patients with elevated serum IgE levels, and 6 atopic dermatitis patients with normal IgE levels. On Barn HI, Hind III, and Eco RI digestions, we detected three hybridizable fragments containing three human C ε genes, C ε 1, C ε 2, and C ε 3, respectively, in all leukocyte DNAs. These fragments were almost identical in size among patients and healthy donors. Pst I digestion generated a genetic polymorphism. We, however, could find no correlation between this polymorphism and the disorders. It was concluded that among the patients and healthy donors, there was no marked difference in the organization of the functional C ε gene and its flanking region containing a class switch region. Our conclusion cannot rule out the presence of genetic abnormalities of this region in some atopic dermatitis patients which are not resolvable by our method. In the course of this study, we found a novel C ε -like gene in placenta DNA which differs from the three C ε genes commonly present in normal human DNA.