The haemagglutinating properties of infectious pancreatic necrosis virus

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Abstract
The haemagglutinating properties of infectious pancreatic necrosis virus have been investigated. Erythrocytes from a wide range of animals were used in these tests but only cells derived from mice (Balb/c and Manchester strain) gave reproducible haemagglutination. The tests were carried out at various temperatures but haemagglutination only occurred at 4° and 22° C. The haemagglutination was pH dependent with an optimum being between pH 5.75 and 6.0. Variable results were obtained at the same pH values with erythrocytes derived from rats (Sprague Dawley) but the reaction in this case appeared to be related to individual batches of erythrocytes.