INDUCTION OF TRANSPLANTATION IMMUNITY BY DANSYLATED TUMOR-CELLS

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 67  (2) , 315-319
Abstract
Tumor cells were coupled with fluorescent dansyl groups in aqueous medium by dansyl chloride-cycloheptaamylose complex (CDC) without destruction of the cells. C57BL/6 mice and Donryu rats pretreated respectively with dansylated EL4 leukemia cells and dansylated Yoshida sarcoma cells acquired transplantation immunity to the corresponding tumor cells. Serum and spleen cells obtained from EL4 immune mice showed cytotoxicity to EL4 cells but not to other allogeneic leukemia cells. Hapten-specific cytotoxicity of immune serum and spleen cells was not observed in the present immune system.