The distribution of guanidines, phosphagens and N-amidino phosphokinases in echinoids

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Abstract
Two echinoids, Centrostephanus rodgersii and Heliocidaris erythrogramma Valenciennes, were examined for the presence of free guanidine, phosphorylated derivatives and the respective N-amidino phosphokinases. C. rodgersii contains arginine, phosphoarginine and arginine phosphokinase. H. erythrogramma, on the other hand, contained both creatine and arginine together with their phosphorylated derivatives and N-amidino phosphokinases. The creatine and arginine phosphokinases in H. erythrogramma are readily separable and are similar in properties to those enzymes isolated from vertebrate and invertebrate sources. Methods for the chromatographic and enzymic characterization of creatine and arginine are described.