The fluorimetric estimation of N1-methylnicotinamide and its differentiation from coenzyme I.
- 1 April 1950
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 46 (4) , 421-426
- https://doi.org/10.1042/bj0460421
Abstract
A rapid and simple method for the estimation of N1-methylnicotinamide was presented. It was modified from the procedure described by Huff et al. (1945) by using methyl ethyl ketone instead of acetone, and by the adoption of 2 alternative blank procedures. The conditions of the reaction and the specificity of the method were studied. With the blank procedures described the fluorescence due to coenzyme I and to N1-methylnicotinuric acid betaine was differentiated from that of N1-methylnicotinamide. Estimations of N1-methylnicotinamide by this method gave values of 4.8 mg./day for human urine, 0.04-0.29 mg./day for rat urine.Keywords
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