Early effect of methacholine on pancreatic RNA synthesis

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Abstract
Previous investigations demonstrate that pancreatic tissue slices from fed pigeons have greater rates of uridine-H3 incorporation into RNA than slices from fasted controls. Fasted pigeons show an increase in uridine-H3 incorporation in pancreatic tissue within 2 hr. of feeding. Considering the well-established secretory effect of cholinergic agents on the pancreas these studies were undertaken to determine whether or not additional metabolic effects might be mediated by such agents. The study indicates that within 5 min following methacholine administration there is an increase in uridine-H3 incorporation by pancreatic tissue slices. The increase in uridine-H3 incorporation following methacholine is blocked by prior actinomycin D administration. Increased L-phenylalanine-C14 incorporation observed following methacholine administration is only partially blocked by actinomycin D.