Spectrophotometric and Fluorimetric Determination of Catecholamines

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Abstract
Spectrophotometric and spectrofluorometric methods have been described for determination of catecholamines in pharmaceutical formulations. These depend upon measuring the intensity of the orange colour produced on reacting catecholamines with the ethanolic solution of o-phenylenediamine dihydrochloride at 490 nm and at 560, 410 nm emission maxima with 1,2-diaminoanthrancene and 2-aminophenol. The absorbance obeys Beer's law in the concentration range of 0.092 to 2.48 mg of catechol amines spectrophotometrically from 4.5 to 240 μg spectrofluorometrically