Determinants ofEscherichia coilRNase P cleavage site selection: a detailedin vitroandin vivoanalysis
- 1 January 1993
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 21 (3) , 427-434
- https://doi.org/10.1093/nar/21.3.427
Abstract
The location of the Escherichia coli RNase P cleavage site was studied both in vitro and in vivo. We show that selection of the cleavage site is dependent on the nucleotide at the cleavage site and the length of the acceptor-stem. Within the acceptor-stem the number of nucleotides on the 5'-half of the acceptor-stem appears to be the important determinant, rather than the number of base pairs in the acceptor-stem. We also demonstrate that the length of the T-stem and a G to C substitution at position 57 in the tRNA(Tyr)Su3 precursor influence the location of the cleavage site under certain conditions. With respect to the function of the subunits of RNase P our data suggest that the nucleotide at position 333 in M1 RNA, and the C5 protein, are important for the identification of the cleavage site.Keywords
This publication has 27 references indexed in Scilit:
- Several regions of a tRNA precursor determine the Escherichia coli RNase P cleavage siteJournal of Molecular Biology, 1992
- Important 2′-hydroxyl groups in model substrates for M1 RNA, the catalytic RNA subunit of RNase P from Escherichia coliJournal of Molecular Biology, 1992
- Ribonuclease P RNA and protein subunits from bacteriaNucleic Acids Research, 1992
- The kinetics and specificity of cleavage by RNase P is mainly dependent on the structure of the amino acid acceptor stemNucleic Acids Research, 1992
- Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templatesNucleic Acids Research, 1987
- A single mutation in loop IV of Escherichia coli SuIII tRNA blocks processing at both 5‘- and 3‘-ends of the precursor tRNA.Journal of Biological Chemistry, 1986
- The RNA moiety of ribonuclease P is the catalytic subunit of the enzymePublished by Elsevier ,1983
- Nucleotide sequence of the gene encoding the RNA subunit (M1 RNA) of ribonuclease P from Escherichia coliCell, 1982
- Correlation of nonsense sites in the lacI gene with specific codons in the nucleotide sequenceNature, 1978
- Three-Dimensional Structure of Yeast Phenylalanine Transfer RNA: Folding of the Polynucleotide ChainScience, 1973