Biochemical Genetic Stock Identification of Pink Salmon (Oncorhynchus gorbuscha) in Southern British Columbia and Puget Sound

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Abstract
We used electrophoresis to examine genetic variability at 12 loci for 4 even-year broodline stocks and at 14 loci for 21 odd-year broodline stocks of pink salmon (Oncorhynchus gorbuscha) in southern British Columbia and Puget Sound. Allelic frequencies were most heterogeneous between the two pink salmon broodlines, but within the odd-year broodline, Fraser River, Canadian non-Fraser, and Puget Sound pink salmon had significantly different allelic frequencies at some loci. Canadian non-Fraser River stocks had the greatest heterogeneity of allelic frequencies within a region, whereas the Fraser River stocks had the least amount of heterogeneity. There was no significant two-locus linkage disequilibrium for the pink salmon stocks surveyed. Cluster analyses by allelic frequencies indicated that Fraser River, Canadian non-Fraser, and Puget Sound stocks were reasonably distinctive. We used differences in genotypic frequencies at 11 loci (3 were duplicated loci) to estimate stock composition of these three groups of pink salmon in mixtures in which the true compositions were known, and we were able to compare the accuracy and precision of our estimates with respect to mixture size and stock composition. We estimated that 100% of pink salmon sampled in a test fishery in Thompson Sound were of Canadian non-Fraser origin.