A rapid urease test for presumptive identification of Cryptococcus neoformans
- 1 October 1996
- journal article
- Published by Springer Nature in Mycopathologia
- Vol. 136 (1) , 21-23
- https://doi.org/10.1007/bf00436656
Abstract
A rapid method to evidence urease activity is described. Urea hydrolysis and consequent production ammonia are detected by a chemical reaction producing a blue phenol compound (indophenol blue). Three hundred and three yeast were tested. Out of 107 urease-positive organisms detected by Christensen's Urea Agar Test (CUAT) 102 were positive by our method. No false negatives were observed by this method when testing 87 Cryptococcus strains. Ths practical screening test for presumptive identification of Cryptococcus neoformans is simple, unaffected by pH changes and requires 15 minutes to be performed.Keywords
This publication has 19 references indexed in Scilit:
- Cryptococcosis in the era of AIDS--100 years after the discovery of Cryptococcus neoformans.1995
- Cryptococcosis in the era of AIDS--100 years after the discovery of Cryptococcus neoformansClinical Microbiology Reviews, 1995
- Cryptococcosis produced by a urease negative strain ofCryptococcus neoformansMedical Mycology, 1993
- The Growing Problem of Mycoses in Patients Infected with the Human Immunodeficiency VirusClinical Infectious Diseases, 1991
- Microbial ureases: significance, regulation, and molecular characterization.1989
- Microbial ureases: significance, regulation, and molecular characterization.Microbiological Reviews, 1989
- Disseminated infection caused by urease-negative Cryptococcus neoformansJournal of Clinical Microbiology, 1988
- Two rapid pigmentation tests for identification of Cryptococcus neoformansJournal of Clinical Microbiology, 1982
- Rapid selective urease test for presumptive identification of Cryptococcus neoformansJournal of Clinical Microbiology, 1979
- Phenol-hypochlorite reaction for determination of ammoniaAnalytical Chemistry, 1967