Conformational Analysis of Serum Apolipoprotein AII in Lipoprotein Complexes with Bifunctional Crosslinking Reagents
- 1 January 1979
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 360 (1) , 691-708
- https://doi.org/10.1515/bchm2.1979.360.1.691
Abstract
Apolipoprotein AII isolated from human serum high density lipoproteins was recombined with [soybean] phosphatidylcholine to yield homogeneous particles of 80-120 .ANG. diameter. The radioactive bifunctional crosslinkers dimethyl [1,1''-14C]suberimidate and dimethyl 4,4''-dithio-bis([1-14C]butyrimidate) were reacted with these particles. The kinetics of the reactions and the localization of the crosslinked Lys of the polypeptide chains were determined. Thermolysin [EC 3.4.24.4] hydrolysis followed by 2-dimensional separation of the peptides and isolation of the mono- and bifunctionally modified peptides allowed the assignment of the crosslinked peptides of the apoprotein AII sequence. The crosslinking pattern indicates a close-neighbor relationship (13-15 .ANG.) of the peptide chains between amino acid residues 3, 23, 46 and 55 of the symmetrical halves of the apo AII molecule. A reconstruction of the secondary structure of Apo AII in the lipoprotein complex on the basis of theoretical calculations is given and correlated with the chemical data.This publication has 30 references indexed in Scilit:
- Covalent Cross-linking of Photosensitive Phospholipids to Human Serum High Density Apolipoproteins (apoHDL)Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1979
- Surface Localization of Apolipoprotein AII in Lipoprotein-ComplexesHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1979
- The amino acid sequence of human Apoa-I, an apolipoprotein isolated from high density lipoproteinsBiochemical and Biophysical Research Communications, 1978
- Chemical Proof of Lipid-Protein Interactions by Crosslinking Photosensitive Lipids to Apoproteins. Intermolecular Cross-Linkage between High-Density Apolipoprotein A-I and Lecithins and SphingomyelinsHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1977
- Lipid-Protein Interactions between Human Apolipoprotein A-I and Defined Sphingomyelin Species. A13C-NMR Spectroscopic StudyHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1977
- A disulfide-bridge bifunctional imidoester as a reversible cross-linking reagentBiochemical and Biophysical Research Communications, 1975
- Studies on the Protein Defect in Tangier DiseaseJournal of Clinical Investigation, 1972
- A Simple Chemical Method for Labelling Phosphatidylcholine and Sphingomyelin in the Choline MoietyHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1971
- Beitrag zur Aminosäurebestimmung auf PapierchromatogrammenHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1958
- The Use of Bunte Salts in Synthesis. II. The Preparation of Derivatives of Mercapto Aliphatic AcidsJournal of the American Chemical Society, 1941