Surface Localization of Apolipoprotein AII in Lipoprotein-Complexes
- 1 January 1979
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 360 (1) , 685-690
- https://doi.org/10.1515/bchm2.1979.360.1.685
Abstract
Lipoprotein particles reconstituted from the apolipoprotein AII (apo AII) component of human serum high density lipoprotein, [soybean] phosphatidylcholine and lysophosphatidylcholine were covalently linked to the imidoester groups of a polystyrene residue. Apo AII was proteolytically digested with thermolysin [EC 3.4.24.4] after delipidation. The covalently bound peptides remaining at the resin were cleaved and separated by combined 2-dimensional electrophoresis/chromatography. The peptides were isolated, hydrolyzed and their amino acid composition determined. They were assigned to the apo AII sequence. Since the imidoester groups on the surface of the resin carrier cannot react with buried Lys residues, this method gives strong chemical evidence for the spreading of the apo AII polypeptide chain over the surface of the lipoprotein particle, as far as the sequence carrying Lys residues between residue 22 and 55 of each symmetrical half is concerned.This publication has 14 references indexed in Scilit:
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