Disposition of a soluble chromate in the isolated perfused rat liver

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Abstract
The uptake of potassium dichromate in the isolated perfused rat liver was almost complete after one hour of perfusion. No significant sex differences in chromium distribution were observed. At the end of the experiments (one hour), about 60% of the applied Cr(VI) dose (312 .mu.g Cr/liver) was located in the cytosol, 14% was in the mitochondria, 9% in the microsomal pellet and 2% was associated with the nuclei. Gel chromatographyof the cytosol showed that most of the chromium was in fractions with an apparent molecular weight of 6000 Da and absorption maxima at 410 nm and 548 nm. Similar optimal properties and molecular weight are characteristic of GSH.sbd.Cr complexes formed by reaction of Cr(VI) GSH in vitro.