The Determination of Catechol Amines in Blood

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Abstract
(1)The effect of light on the fluorescence of the ethylene diamine condensation products of the oxidized catechol amines has been studied. (2)It was found that exposure of the norepinephrine derivative for 30 minutes to light reduced the fluorescence to a very low level. The epinephrine complex, on the other hand, is only slightly affected by the same exposure. (3)On the basis of this differential sensitivity to light a sensitive method for the quantitative determination of the two catechol amines in blood has been developed. (4)The levels of epinephrine and norepinephrine in the blood of normal individuals were found to range from 0.1-1.5 µg per liter and 0.3-4.0 µg per liter, respectively. (5)Further work must be done before this method can be applied to the determination of the catechol amines in urine.

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