In vitro Response of Mouse Testis to Human Chorionic Gonadotropin.

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Abstract
Testes from 3-day-old mice were cultured in an in vitro system for 7 or 14 days on a medium composed of agar, Tyrode''s solution, synthetic medium 199 and chick embryo extract. Degenerative changes in control explants included a decrease in the size of the tubules and a reduction in the cytoplasm of the interstitial cells. Inclusion of human chorionic gonadotropin (HCG) in the medium ameliorated these changes. Moreover, measurable amounts of androgen were produced by testes cultured on media enriched with HCG whereas no detectable amount was produced by those cultured on media enriched with lactogenic hormone.