EFFECT OF DIBUTYRYL CYCLIC AMP AND OESTRADIOL ON INCORPORATION OF [3H]LEUCINE INTO THE PROTEINS OF LUMINAL FLUID OF THE RAT UTERUS
- 1 September 1977
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 74 (3) , 431-439
- https://doi.org/10.1677/joe.0.0740431
Abstract
SUMMARY: An injection of 12·5 nmol dibutyryl cyclic AMP (dbcAMP) into each uterine horn of ovariectomized rats maintained on progesterone alone, enhanced incorporation of [3H]leucine into uterine luminal proteins. A value of 11·73 × 103 c.p.m. [3H]leucine incorporated/ rat was found after injection of dbcAMP compared with 3·69 × 103 c.p.m./rat after injection of saline. An injection of 1·0 μg oestradiol, in contrast, gave 58·05 × 103 c.p.m./rat. Electrophoretic analysis showed that the radioactively labelled proteins found after an injection of dbcAMP or oestradiol were qualitatively similar to those detected on day 5 of pregnancy, the day of blastocyst implantation. The nucleotide thus apparently elicits a uterine response similar to that observed after an injection of oestradiol.This publication has 1 reference indexed in Scilit:
- Cyclic AMP and the preparation of the mouse uterus for implantationReproduction, 1977