Spermine-DNA complexes build up metastable structures. Small-angle X-ray scattering and circular dichroism studies
- 1 January 1979
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 7 (5) , 1297-1310
- https://doi.org/10.1093/nar/7.5.1297
Abstract
Spermine-DNA complexes have been examined by small-angle and wide-angle X-ray scattering as well as by circular dichroism studies. Condensed complexes are building up below a critical ionic strength. We have found that at one and the same ionic strength condensed complexes having two different supramolecular structures (Type I and Type II) can coexist. The structure of the condensates depends on the method of condensate formation. Phase transitions between these structures can be induced by thermal treatment. We conclude from these facts that with polyamine-DNA condensates metastable structures are of importance.Keywords
This publication has 11 references indexed in Scilit:
- Study of DNA-spermine interactions by use of small-angle and wide-angle X-ray scattering and circular dichroismNucleic Acids Research, 1978
- DNA condensation with polyaminesJournal of Molecular Biology, 1978
- DNA condensation with polyaminesJournal of Molecular Biology, 1978
- The compaction of DNA helices into either continuous supercoils or folded-fiber rods and toroidsCell, 1978
- SECONDARY STRUCTURE OF CONDENSED DNA - WIDE ANGLE, SMALL-ANGLE X-RAY-SCATTERING, AND CIRCULAR-DICHROISM STUDIES1978
- Significant changes in 16 S RNA conformation accompanying assembly of the 30 S ribosome in vitroJournal of Molecular Biology, 1977
- Polyamine induced aggregation of DNANucleic Acids Research, 1977
- A Transition to a Compact Form of DNA in Polymer SolutionsProceedings of the National Academy of Sciences, 1971
- CRYSTALLIZATION OF DNA FROM DILUTE SOLUTIONProceedings of the National Academy of Sciences, 1969
- Micro diffusion cells for the growth of single protein crystals by means of equilibrium dialysisArchives of Biochemistry and Biophysics, 1968