Practical Considerations in the Measurement of Culture Fluorescence
- 5 September 1990
- journal article
- research article
- Published by Wiley in Biotechnology Progress
- Vol. 6 (5) , 398-401
- https://doi.org/10.1021/bp00005a013
Abstract
We have examined practical considerations associated with the use of a commercially available fluorescence probe for in situ measurements in bioreactors. The optical path length of the measurement was first determined and a flow cell subsequently designed. The environment (agitation/aeration rates) of the probe was found to have a significant influence on the measurement. These effects were eliminated by placing the probe in a recycle loop using the flow cell. Fluorescence measurement in the recycle loop was verified to be representative of the cell sample and to not affect cell metabolism.This publication has 9 references indexed in Scilit:
- Analysis of a fermentation recycle loop for on-line measurementsBiotechnology Techniques, 1990
- Use of culture fluorescence as a sensor for on-line discrimination of host and overproducing recombinantEscherichia coliBiotechnology & Bioengineering, 1989
- NADH levels and solventogenesis in Clostridium acetobutylicum: New insights through culture fluorescenceApplied Microbiology and Biotechnology, 1989
- Inner filter effects and their interferences in the interpretation of culture fluorescenceBiotechnology & Bioengineering, 1987
- THE INTERPRETATION OF ON-LINE PROCESS MEASUREMENTS OF INTRACELLULAR NADH IN FERMENTATION PROCESSESChemical Engineering Communications, 1986
- On-line measurements of culture fluorescence: Method and applicationApplied Microbiology and Biotechnology, 1981
- Estimation of Fermentation Biomass Concentration by Measuring Culture FluorescenceApplied and Environmental Microbiology, 1978
- Fluorimetric Technique for Monitoring Changes in the Level of Reduced Nicotinamide Nucleotides in Continuous Cultures of MicroorganismsApplied Microbiology, 1970
- Concentrations of Nicotinamide Nucleotide Coenzymes in Micro-OrganismsJournal of General Microbiology, 1966