PCR-Restriction Fragment Length Polymorphism Analysis of a Diagnostic 452-Base-Pair DNA Fragment Discriminates between Cryptosporidium parvum and C. meleagridis and between C. parvum Isolates of Human and Animal Origin
Open Access
- 1 April 2002
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 68 (4) , 2071-2076
- https://doi.org/10.1128/aem.68.4.2071-2076.2002
Abstract
Genomic DNAs from human Cryptosporidium isolates previously typed by analysis of the 18S ribosomal DNA locus ( Cryptosporidium parvum bovine genotype, C. parvum human genotype, Cryptosporidium meleagridis , and Cryptosporidium felis ) were used to amplify the diagnostic fragment described by Laxer et al. (M. A. Laxer, B. K. Timblin, and R. J. Patel, Am. J. Trop. Med. Hyg., 45:688-694, 1991). The obtained 452-bp amplified fragments were sequenced and aligned with the homologous Cryptosporidium wrairi sequence. Polymorphism was exploited to develop a restriction fragment length polymorphism method able to discriminate Cryptosporidium species and C. parvum genotypes.Keywords
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