Isolation and characterization of the porcine nuclear factor I (NFI) gene
- 15 August 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 236 (1) , 27-32
- https://doi.org/10.1016/0014-5793(88)80279-5
Abstract
This study describes the isolation of a major portion of the gene for nuclear factor I (NFI) including its 5′‐flanking region with transcriptional start sites. We screened a porcine liver, genomic DNA library in phage EMBL3A with synthetic oligonucleotides derived from tryptic and cyanogen‐bromide peptide sequences obtained from purified NFI protein. The NFI gene is present as a single copy in porcine DNA.Keywords
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