CULTURED HUMAN-ENDOTHELIAL CELLS DISPLAY AN ANTIGEN THAT IS RECOGNIZED BY CERTAIN HUMAN ANTI-CHROMATIN AUTOANTIBODIES

  • 1 January 1983
    • journal article
    • research article
    • Vol. 54  (2) , 373-377
Abstract
Certain human anti-nucleosome autoantibodies cross-reacted specifically with an antigen on the surface of human vascular endothelial cells. This was shown by 2 different techniques. Antibodies eluted from the surface of cultured endothelial cells bound to cell nuclei; this binding was inhibited by soluble mononucleosomes. X-ANA [cross-reacting antinuclear antibodies], specifically isolated by their affinity to polynucleosomes, stained the plasma membranes of single cell suspensions of endothelial cells in the indirect immunofluorescence test. Several lines of evidence excluded Fc receptor-mediated binding of Ig to the cells. Plasma membranes of vascular endothelial cells and chromatin bear an antigen that is serologically related.

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