Genetic studies on the β subunit of Escherichia coli RNA polymerase
- 1 December 1982
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 188 (3) , 399-404
- https://doi.org/10.1007/bf00330040
Abstract
The use of five different nonsense suppressors, in conjunction with a collection of 95 independent, spontaneously-occurring amber mutants affecting expression of rpoB, allows the generation of a maximum of 475 potential variants of the β subunit of E. coli RNA polymerase, each carrying a known amino acid substitution at a particular site. The effect of these amino acid exchanges has been investigated in vivo. A significant majority (363/475) of substitutions lead to cellular death and altered properties —temperature sensitivity, apparently altered transcription termination and a changed stringent response—indicating that RNA polymerase function (unlike that of dispensible proteins) is extremely sensitive to such single site changes.This publication has 51 references indexed in Scilit:
- In vivo synthesis of a polycistronic messenger RNA for the ribosomal proteins L11, L1, L10 and L7/12 in Escherichia coliMolecular Genetics and Genomics, 1981
- Mutagenic deamination of cytosine residues in DNANature, 1980
- The influence of codon context on genetic code translationNature, 1980
- Molecular basis of base substitution hotspots in Escherichia coliNature, 1978
- Control of Transcription TerminationAnnual Review of Biochemistry, 1978
- Interaction between mutations of ribosomes and RNA polymerase: a pair of strA and rif mutants individually temperature-insensitive but temperature-sensitive in combination.Proceedings of the National Academy of Sciences, 1977
- Variations in the rate of synthesis of β and β′ RNA polymerase polypeptides under the influence of certain factorsMolecular Genetics and Genomics, 1977
- Suppressor su+7 inserts tryptophan in addition to glutamineJournal of Molecular Biology, 1976
- Reference mutations for the β subunit of RNA polymeraseMolecular Genetics and Genomics, 1974
- Amber Mutations of Escherichia coli RNA PolymeraseNature New Biology, 1971