Development of Antibody to Growth Hormone–Releasing Factor

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Abstract
To the Editor: In a recent paper in the Journal (Jan. 3 issue)1 we reported the acceleration of growth in two children treated with human growth hormone–releasing factor. We stated that when a protein A separation technique was used, all the serum samples drawn from both children over the six months of therapy had less than twice the nonspecific binding of 125I-growth hormone–releasing factor. Since protein A selectively binds IgG1, IgG2, and IgG4, but not IgG3,2 we repeated the experiments using goat antihuman IgG raised against crude IgG. This antibody is polyclonal . . .