Tissue fractionation studies. 16. Intracellular distribution and properties of α-glucosidases in rat liver

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Abstract
The intracellular distribution of rat-liver [alpha]-(l[forward arrow] 4)-glucosidases (maltases) has been investigated by a fractionation scheme in which the mitochondrial fraction is divided into subtractions. An acid maltase was found to be concentrated in the lysosome-rich light-mitochondrial fraction, whereas neutral-maltase activity was found in both the microsomal and the soluble fractions. The lysosomal localization of acid maltase has been confirmed by density equilibration in a density gradient, by its structure-linked latency and by its release under controlled damage. Some kinetic properties of the rat-liver maltases have been investigated.