Spectrophotometric Method for Determination of Heat-Activated Sulfhydryl Groups of Skimmilk
Open Access
- 1 February 1968
- journal article
- research article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 51 (2) , 217-219
- https://doi.org/10.3168/jds.s0022-0302(68)86956-5
Abstract
The activated sulfhydryl (-SH) values of heated skimmilk were determined spectro-photometrically by the use of 5,5''dithiobis(2-nitrobenzoic acid) [DTNB] reagent. To the cooled milk sample was added the diluted DTNB reagent, distilled water; pH 8.0, phosphate buffer; and ammonium sulfate. The mixture was filtered through Whatman 42 filter paper and the absorbance of the filtrate was read at 412 m[mu]. Results obtained were consistent with other currently used procedures. Kinetic values obtained by the method are given.This publication has 8 references indexed in Scilit:
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