High-efficiency receptor-mediated delivery of small and large (48 kilobase gene constructs using the endosome-disruption activity of defective or chemically inactivated adenovirus particles.
- 1 July 1992
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (13) , 6094-6098
- https://doi.org/10.1073/pnas.89.13.6094
Abstract
One limit to successful receptor-mediated gene delivery is the exit of the endocytosed material from the endosome. We demonstrate here the delivery of marker genes to tissue culture cells using a modification of the receptor-mediated gene delivery technique that exploits the endosomolytic activity of defective adenovirus particles. In particular, greater than 90% of the transfected-cell population is found to express a beta-galactosidase gene, and, most importantly, this high level of expression can be obtained with psoralen-inactivated virus particles. Furthermore, because the delivered gene is not carried within the genome of the adenovirus particle, the size constraints are relieved, and we can, therefore, show the delivery of a 48-kilobase cosmid DNA molecule.Keywords
This publication has 31 references indexed in Scilit:
- High-Efficiency Gene Transfer Mediated by Adenovirus Coupled to DNA–Polylysine ComplexesHuman Gene Therapy, 1992
- In vivo transfer of the human cystic fibrosis transmembrane conductance regulator gene to the airway epitheliumCell, 1992
- DNA-binding transferrin conjugates as functional gene-delivery agents: synthesis by linkage of polylysine or ethidium homodimer to the transferrin carbohydrate moietyBioconjugate Chemistry, 1991
- Evaluation of the Transfer and Expression in Mice of an Enzyme-Encoding Gene Using a Human Adenovirus VectorHuman Gene Therapy, 1990
- STRUCTURE AND ASSEMBLY OF COATED VESICLESAnnual Review of Biophysics, 1987
- Expression of a foreign gene in myeloid and lymphoid cells derived from multipotent haematopoietic precursorsNature, 1985
- The psoralen-DNA photoreaction. Characterization of the monoaddition products from 8-methoxypsoralen and 4,5',8-trimethylpsoralenBiochemistry, 1982
- Viral infection permeabilizes mammalian cells to protein toxinsCell, 1980
- Model nucleohistones: The interaction of F1 and F2al histones with native T7 DNAJournal of Molecular Biology, 1971
- Deoxyribonucleic acid-polylysine complexes. Structure and nucleotide specificityBiochemistry, 1969