Species differences in the conjugation of free bile acids with taurine and glycine

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Abstract
The conjugation of free bile acids with taurine and glycine performed by rat, rabbit and chicken liver microsomes was studied. Rat-liver microsomes are shown to conjugate free bile adds with both glycine and taurine, but taurine is preferred in the conjugation. Rabbit-liver microsomes conjugate the free bile acids almost exclusively with glycine. Chicken-liver microsomes conjugate cholic acid exclusively with taurine. Cholic, deoxycholic, 3:7:12-trioxo-cholanic and cholanic acids have been shown to serve as substrates in the conjugation, both in the rat and rabbit. Trioxocholanic acid was shown to be less efficient than the cholic and deoxycholic acids. Tauro-cholic and glycocholic acids were shown to inhibit the conjugation by preventing the formation of cholyl-S-CoA, presumably by acting as antimetabolite to coenzyme A. Results are discussed in relation to cholesterol metabolism.