Site of tubulin synthesis in Tetrahymena pyriformis
- 25 July 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 158 (2) , 271-275
- https://doi.org/10.1016/0014-5793(83)80593-6
Abstract
Free and membrane‐bound polysomes were isolated from the protozoa Tetrahymena pyriformis, and the contribution of these two types of polysomes to tubulin synthesis were studied using immunoprecipitation of the 35S‐translational products in a rabbit reticulocyte lysate. One‐dimensional electrophoretic analysis shows that tubulin is synthesized by polyadenylated RNA isolated from free and membrane‐bound polysomes. Non‐polyadenylated RNAs of free polysomes are also able to direct tubulin synthesis. Two‐dimensional electrophoretic analysis using O'Farrell's system confirms these results and also reveals the existence of the α‐ and β‐tubulin subunits.Keywords
This publication has 18 references indexed in Scilit:
- Post-translational modification of tubulin dependent on organelle assemblyNature, 1982
- Evolution of tubulin heterogeneity during mouse brain developmentBiochimie, 1982
- α-tubulin genes of drosophilaCell, 1981
- Non‐polyadenylated mRNAs from eukaryotesFEBS Letters, 1980
- Translation in vitro of Tetrahymena pyriformis Polyadenylated mRNAEuropean Journal of Biochemistry, 1979
- Isolation of separate mRNAs for α- and β-Tubulin and characterization of the corresponding in vitro translation productsCell, 1978
- The function of tubulin in motile systemsBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1976
- Rapidly Labelled RNA in Tetrahymena pyriformisEuropean Journal of Biochemistry, 1975
- MicrotubulesAnnual Review of Biochemistry, 1973
- Isolated cilia from Tetrahymena pyriformisExperimental Cell Research, 1962