Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 61 (4) , 335-344
- https://doi.org/10.1007/bf00288617
Abstract
Total cytoplasmic polyadenylated RNA was isolated from the human lymphocyte cell line Wil2 by oligo(dT)-cellulose chromatography. Tritiated complementary DNA (cDNA) was transcribed from the RNA and used as a probe for in situ hybridization to metaphase chromosomes. The majority of G-negative or lightly staining bands were found to be preferential sites of hybridization.Keywords
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