Hyperglycemic-Glycogenolytic Factor in Diabetic Man and Alloxan-Diabetic Animals

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Abstract
HGF was isolated from the urine of alloxan diabetic rats and rabbits and from the urine of diabetic patients by precipitating in 72-80% alcohol, redissolving in phosphate buffer at pH4-4.5, and the dialyzed and non-dialyzed parts evaporated by vacuum. HGF was isolated from normal pancreases by the same technic. Intraven. injn. of HGF in a normal rabbit and perfusion expts. in the liver or, in vitro, in liver slices showed the inhibitory or reversing effects of different substances, as peptic digestion, alkaline and insulin, on HGF. Finally the pathogeny of diabetes and the role of alpha cells in pancreas was discussed based on the current view on this subject.