Complementary DNA derived structure of the amino-terminal domain of human apolipoprotein B and size of its messenger RNA transcript
- 23 September 1986
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 25 (19) , 5351-5357
- https://doi.org/10.1021/bi00367a001
Abstract
In this paper the sequence of a 5.2-kilobase (kb) cDNA covering the amino-terminal domain of human apolipoprotein B-100 (apoB-100) is reported. The cDNA-derived protein sequence provides the primary structure of 1748 amino acids. This segment of apoB-100 is more hydrophilic than hydrophobic and contains short stretches of predicted helical and .beta. structures that are interrupted by .beta. turns. Blotting analysis of RNA isolated from fetal human and adult monkey tissues and various human cell lines showed synthesis of apoB mRNA by liver and intestine and by cells of hepatic (HepG2) and intestinal (Caco-2) origin. The isolation and characterization of overlapping cDNA clones, which provide a nearly full-length copy of human apoB-100, are also reported. From the length of these clones the size of the cytoplasmic apoB mRNA is estimated to be 14.0 kb and codes for a protein of appproximately 512000 daltons.This publication has 27 references indexed in Scilit:
- Synthesis, processing, and secretion of apolipoprotein B by the chick liver cell.Journal of Biological Chemistry, 1982
- Properties of apolipoprotein b in urea and in aqueous buffers the use of glutathione and nitrogen in its solubilizationBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1981
- Normotriglyceridemic abetalipoproteinemia. absence of the B-100 apolipoprotein.Journal of Clinical Investigation, 1981
- Variant forms of plasma apolipoprotein B. Hepatic and intestinal biosynthesis and heterogeneous metabolism in the rat.Journal of Biological Chemistry, 1981
- Immunoregulatory plasma lipoproteins. Role of apoprotein E and apoprotein B.Journal of Biological Chemistry, 1980
- Heterogeneity of apolipoprotein B: isolation of a new species from human chylomicrons.Proceedings of the National Academy of Sciences, 1980
- Interaction of plasma lipoproteins containing apolipoproteins B and E with heparin and cell surface receptorsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1979
- Peptide chain conformation and the glycosylation of glycoproteinsBiochemical and Biophysical Research Communications, 1977
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- Serum Cholesterol, Lipoproteins, and the Risk of Coronary Heart DiseaseAnnals of Internal Medicine, 1971