Genome Analysis of BK (WW) Viral DNA Cloned Directly from Human Urine
- 1 January 1984
- journal article
- research article
- Published by S. Karger AG in Intervirology
- Vol. 22 (3) , 170-176
- https://doi.org/10.1159/000149547
Abstract
DNA from a 3rd isolate of the WW strain of BK virus was prepared from human urine and was cloned into the BamHI site of pBR322. Restriction mapping showed a number of changes relative to the prototype BK viral DNA, including the presence of a new PstI, an SstI and an AvaII site and the loss of a HindIII an SstI, and an NcoI site. There is evidence for more extensive changes in the vicinty of the origin of replication.Keywords
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