COMPARISON OF ZYMOGRAM OF 3 LYMPHOBLASTOID CELL LINES WITH A NEW MICROTECHNIQUE

  • 1 January 1976
    • journal article
    • research article
    • Vol. 24  (3) , 183-190
Abstract
The enzymatic activities of 3 lymphoblastoid cell lines were subjected to comparative analysis of 22 enzymatic activities with a new microtechnique Apizym. This method is easy, requires only a small number of cells and allows simultaneous testing of many enzymatic activities from the same sample. The 3 cell lines can be easily distinguished despite their close origin: NAB, derived from a malignant retroperitoneal mass in an American patient, is the cell line with the greatest total activity and HRIK (a clone of P3J, derived from an African lymphoma) with the lowest and RAJI (a nonvirus producing line derived from an African Burkitt lymphoma) being intermediate activity. The more striking differences concern the activity of phosphatases (NAB > RAJI .gtoreq. HRIK), esterases-lipases (NAB .gtoreq. RAJI > HRIK), .beta.-glucosaminidase, .beta.-glucuronidase and .beta.-glucosidase (NAB .gtoreq. RAJI .BETA. HRIK). .alpha.-Mannosidase and .alpha.-fucosidase are more active in RAJI (RAJI > NAB .gtoreq. HRIK).