Induction of self-tolerance and enhanced stress protein synthesis in L-132 cells by cadmium chloride and by hyperthermia

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Abstract
The effect of heat shock or cadmium treatment on protein synthesis and cell survival in L-132 cells has bene examined. After cadmium treatment, the synthesis of a polypeptide of MR 68000 (P68) was greatly enhanced over that of untreated cells. Besides P68 the sythesis of another polypeptide of MR 89000 (P89) was also enhanced in heat shocked cells. Both heat shock and cadmium treatment induced self-tolerance. The kinetics of the synthesis induced self-tolerance. The kinetics of the synthesis of induced polypeptides correlated well with the development of self-tolerance. The patterns of peptide maps obtained after partial proteolytic digestion from P68 induced in cadmium-treated and heat-shocked cells were virtually identical. However, heat-shocked cells did not confer cadmium tolerance nor did cadmium-treated cells induce thermotolerance.