LOSS OF THE SAME ALLELES OF HRAS1 AND D11S151 IN 2 INDEPENDENT PANCREATIC CANCERS FROM A PATIENT WITH MULTIPLE ENDOCRINE NEOPLASIA TYPE-1
- 15 May 1989
- journal article
- research article
- Vol. 49 (10) , 2716-2721
Abstract
The DNAs from two independent pancreatic cancers (tumors 1 and 2) in a patient with multiple endocrine neoplasia type 1 were analyzed. No amplification or gross rearrangement of 19 protooncogenes was observed. However, Southern blot analysis using polymorphic DNA probse revealed loss of heterozygosity at loci on chromosome 11p in both tumors. In tumor 1, an extensive region including the HRAS1, PTH, CALCA, and D11S151 loci was deleted, while in tumor 2 loss of heterozygosity was limited at the HRAS1 and D11S151 loci. Because loss of heterozygosity at other chromosomal loci in the two tumors was quite rare, loss of genes on 11p might be nonrandom. It is noteworthy that the same allele at the HRAS1 locus and also the same allele at the D11S151 locus were lost in the two independent tumors. These results suggest that loss of genes in the HRAS1 and/or D11S151 loci plays an important role unmasking the remaining sequences probably having a recessive mutation.This publication has 7 references indexed in Scilit:
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