Ultracentrifuge Lipoprotein Pattern of Serum of Normal, Hypertensive and Hypothyroid Animals

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Abstract
The serum lipoprotein pattern of normal chickens, guinea pigs, rats, rabbits, opossum, monkey, humans, cats, and dogs was detd. at a density of 1.21 and 1.063 in the analytical ultracentrifuge. All sera examined at a density of 1.21, except guinea pig, contained lipoproteins with flotation rates from -S 1 to 15. Cat and dog sera contained no lipoproteins with a rate faster than 23. Those up to the -S 30 class were present in rat, opossum, sheep, monkey and some human sera. Lastly, the 35 or greater classes characterize guinea pig, chicken, rabbit, some monkey and human sera. Lipoproteins with flotation rates of -S 1-15 have an electrophoretic mobility of alpha-1 globulin; the 20-25 class alpha-2 globulin and the 25-40 beta-1 globulin. The concn. of the lipoproteins varied greatly in different spp. Those of the -S 1-15 class in cat, dog, opossum and monkey sera were greater than 200 mg./100 ml. In human sera they were about 150 mg./lOO ml., in rabbit and rat about 100 mg., and in sheep about 40 mg. The 15-40 classes in rat were of the order of 12 mg; rabbit, sheep, opossum, cat and dog, 50 mg./lOO ml.; human and monkey greater than 200 mg./lOO ml. Arterial hypertension following buffer nerve section in dogs resulted in little or no change in the lipoprotein pattern. The pattern of exptl. renal hypertensive dogs showed increased concn. of the -S1 2120-30, which frequently appeared as a double peak. The appearance of increased concn. of alpha-2 lipoprotein (-S 23) and increase in gamma globulin differentiated exptl. renal from neurogenic hypertension. Admn. of diets high in cholesterol to dogs and rats with thyroid glands inactivated by treatment with radioactive I resulted in greatly increased concn. of lipoproteins with flotation rates greater than -S 23. This was correctable by feeding desiccated thyroid. It is probable that the type of normally occurring serum lipoprotein may be an important factor in determining the differences in susceptibility to atheroma in different spp. and individuals. Those with large amts. with flotation rates of -S 3? or faster are most susceptible.

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