ANTIBODY-DEPENDENT CELL-MEDIATED CYTO-TOXICITY AND SPONTANEOUS CELL-MEDIATED CYTO-TOXICITY AGAINST CELLS INFECTED WITH PORCINE TRANSMISSIBLE GASTROENTERITIS VIRUS

  • 1 January 1983
    • journal article
    • research article
    • Vol. 47  (3) , 298-303
Abstract
The objective of this study was to determine whether porcine peripheral blood leukocytes and intestinal intraepithelial leukocytes can mediate antibody-dependent cell-mediated cytotoxicity and spontaneous cell-mediated cytotoxicity against target cells infected with transmissible gastroenteritis virus. Peripheral blood leukocytes collected from 6 young adult pigs and intraepithelial leukocytes from a further 5 pigs were used as effector cells in Cr release assays against pig kidney PK-15 cells persistently infected with transmissible gastroenteritis virus. Both peripheral blood leukocytes and intraepithelial leukocytes were capable of mediating antibody-dependent cell-mediated cytotoxicity and spontaneous cell-mediated cytotoxicity against PK-15 transmissible gastroenteritis cells. While the peripheral blood leukocytes mediated lower levels of specific 51Cr release in spontaneous cell-mediated cytotoxicity than in antibody-dependent cell-mediated cytotoxicity, the intraepithelial leukocytes were more effective in spontaneous cell-mediated cytotoxicity than antibody-dependent cell-mediated cytotoxicity.