Intracellularly Injected Cobaltous Ions Accumulate at Synaptic Densities
- 28 March 1980
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 207 (4438) , 1477-1479
- https://doi.org/10.1126/science.6987734
Abstract
Physiologically identified neurons in the locust were iontophoretically injected with a mixture of cobaltous and potassium ions. After being fixed for electron microscopy, 2.5-micrometer sections of the epoxy-embedded ganglia were intensified with silver. The intensified material was resectioned and examined in the electron microscope. The cobalt-silver precipitate appeared as discrete densities. Localized accumulations of the precipitate were seen within the injected cell along the neuronal membranes and especially at synapses. Location and recognition of the stained neuron in the electron microscope was facilitated by the tendency of the cobaltous ions to aggregate at the synaptic sites.Keywords
This publication has 31 references indexed in Scilit:
- Electron microscopic studies of serially sectioned cat spinal α‐motoneurons. I. Effects of microelectrode impalement and intracellular staining with the fluorescent dye “procion yellow”Journal of Comparative Neurology, 1979
- The physiological characteristics of the primary sensory neurons of the complex tibial organ ofDecticus verrucivorus L. (Orthoptera, Tettigonioidae)Journal of Comparative Physiology A, 1978
- The synaptic architecture of neurons in opossum somatic sensory-motor cortex: A combined anatomical and physiological studyJournal of Neurocytology, 1978
- A silver intensification method for cobalt-filled neurones in wholemount preparationsBrain Research, 1977
- The shapes of sensory and motor neurones and the distribution of their synapses in ganglia of the leech: a study using intracellular injection of horseradish peroxidaseProceedings of the Royal Society of London. B. Biological Sciences, 1976
- The distribution of the Ca++-dependent protein activator of cyclic nucleotide phosphodiesterase in invertebratesBiochemical and Biophysical Research Communications, 1975
- Neuron Geometry and Circuitry via the Electron Microscope: Intracellular Staining with Osmiophilic PolymerScience, 1973
- Neural mechanisms underlying behavior in the locust Schistocerca gregaria I. Physiology of identified motorneurons in the metathoracic ganglionJournal of Neurobiology, 1973
- THE DISTRIBUTION OF SYNAPSES ON A PHYSIOLOGICALLY IDENTIFIED MOTOR NEURON IN THE CENTRAL NERVOUS SYSTEM OF THE LEECHThe Journal of cell biology, 1972
- The cytochemistry of synaptic densities. I. An analysis of the bismuth iodide impregnation methodJournal of Ultrastructure Research, 1971