Identification ofCandida albicans, otherCandidaspecies andTorulopsis glabrataby means of immunofluorescence
- 1 January 1967
- journal article
- Published by Oxford University Press (OUP) in Medical Mycology
- Vol. 5 (4) , 323-328
- https://doi.org/10.1080/00362176785190591
Abstract
Specific identification of Candida albicans type A, C. albicans type B, C. tropicalis, C. stellatoidea, and Torulopsis glabrata was effected by means of a pair of fluorescein-labelled immune globulins directed respectively against C. albicans A and T. glabrata. The former, when absorbed with cells of C. stellatoidea, differentiated C. albicans A - C. tropicalis and C. albicans B - C. stellatoidea from all other yeasts tested, while the latter, absorbed with C. albicans A, separated the 2 pairs into their respective components and also identified T. glabrata. The results of immunofluorescence and conventional methods agreed with each other on the identification of more than 95% of 585 routine yeastlike isolates, of which 335 were C. albicans A and 55 C. albicans B. On a réalisé l'identification spécifique de Candida albicans type A, C. albicans type B, C. tropicalis, C. stellatoidea et Torulopsis glabrata au moyen de deux préparations d'immuno-globulines anti-C. albicans A et anti-T. glabrata marquées à la fluorescéine. La première lorsqu'elle a été absorbée par des cellules de C. stellatoidea, a permis de différencier C. albicans A-C. tropicalis et C. albicans B-C. stellatoidea de toutes les autres levures étudiées. La seconde absorbée par C. albicans A a permis de séparer les deux groupes en leurs composants respectifs et aussi d'identifier T. glabrata. Les résultats des méthodes d'immunofluorescence et de sérologie classique s'accordent sur l'identification de plus de 95% des 585 souches de champignons levuriformes isolées couramment; parmi ceux-ci 335 appartenaient à C. albicans A et 55 à C. albicans B.Keywords
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