Hydrogen autotrophy of Nocardia opaca strains is encoded by linear megaplasmids
- 1 June 1990
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 136 (6) , 1145-1151
- https://doi.org/10.1099/00221287-136-6-1145
Abstract
Several linear megaplasmids were detected in the facultatively lithoautotrophic Gram-positive bacterium Nocardia opaca. The wild-type strain MR11 contains, in addition to the cccDNA plasmids pHG31-aand pHG31-b, the linear plasmids pHG201 (270 kb), pHG202 (400 kb) and pHG203 (420 kb), pHG205 (280 kb) and pHG206 (510 kb). After preparation of DNA from cells embedded in agarose, the linear plasmids were demonstrated by pulsed-field electrophoresis. By means of DNA probes for genes of soluble hydrogenase and riboulose-bisphosphate carboxylase, the conjugative plasmids pHG201 and pHG205 were shown to be the carriers of the genetic information for these enzymes. A restriction map of pHG201 for the enzymes AsnI, SpeI, XbaI is presented.This publication has 13 references indexed in Scilit:
- Genes of lithoautotrophic metabolism are clustered on the megaplasmid pHG1 in Alcaligenes eutrophusMolecular Genetics and Genomics, 1987
- No Correlation Exists between the Conjugative Transfer of the Autotrophic Character and That of Plasmids in Nocardia opaca StrainsMicrobiology, 1986
- Transposition of structural genes to an expression sequence on a linear plasmid causes antigenic variation in the bacterium Borrelia hermsiiNature, 1985
- Nucleotide sequence of the ribulosebisphosphate carboxylase gene from Rhodospirillum rubrumMolecular Genetics and Genomics, 1984
- Physical evidence for plasmids in autotrophic, especially hydrogen-oxidizing bacteriaArchiv für Mikrobiologie, 1982
- Plasmids required for utilization of molecular hydrogen by Alcaligenes eutrophusArchiv für Mikrobiologie, 1981
- Naturally occurring genetic transfer of hydrogen-oxidizing ability between strains of Alcaligenes eutrophusJournal of Bacteriology, 1981
- Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.Proceedings of the National Academy of Sciences, 1979
- A LINEAR PLASMID-LIKE DNA IN STREPTOMYCES SP. PRODUCING LANKACIDIN GROUP ANTIBIOTICSThe Journal of General and Applied Microbiology, 1979
- A procedure for the isolation of deoxyribonucleic acid from micro-organismsJournal of Molecular Biology, 1961