Maternally expressed PGK-Cre transgene as a tool for early and uniform activation of the Cre site-specific recombinase
- 1 January 1998
- journal article
- research article
- Published by Springer Nature in Transgenic Research
- Vol. 7 (2) , 105-112
- https://doi.org/10.1023/a:1008868325009
Abstract
A transgenic mouse strain with early and uniform expres sion of the Cre site-specific recombinase is described. In this strain, PGK-Crem, Cre is driven by the early acting PGK-1 promoter, but, probably due to cis effects at the integration site, the recombinase is under dominant maternal control. When Cre is transmitted by PGK-Crem females mated to males that carry a reporter transgene flanked by loxP sites, even offspring that do not inherit PGK-Cre delete the target gene. It follows that in the PGK-Crem female Cre activity commences in the diploid phase of oogenesis. In PGK-Crem crosses complete recombination was observed in all organs, including testis and ovary. We prepared a mouse stock that is homozygous for PGK-Crem and at the albino (c) locus. This strain will be useful for the early and uniform induction of ectopic and dominant negative mutations, for the in vivo removal of selective elements from targeted mutations and in connection with the manipulation of targeted loci in 'knock in' and related technologiesKeywords
This publication has 17 references indexed in Scilit:
- Know Your Neighbors: Three Phenotypes in Null Mutants of the Myogenic bHLH Gene MRF4Cell, 1996
- Chromosome engineering in miceNature, 1995
- Acre-transgenic mouse strain for the ubiquitous deletion ofloxP-flanked gene segments including deletion in germ cellsNucleic Acids Research, 1995
- Murine PGK‐1 promoter drives widespread but not uniform expression in transgenic miceDevelopmental Dynamics, 1994
- Deletion of a DNA Polymerase β Gene Segment in T Cells Using Cell Type-Specific Gene TargetingScience, 1994
- Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targetingCell, 1993
- Isolation and characterization of a novel cDNA clone encoding a homeodomain that is developmentally regulated in the ventral forebrainNeuron, 1991
- MECHANISMS OF X-CHROMOSOME REGULATIONAnnual Review of Genetics, 1988
- Cloning and expression of the mouse pgk-1 gene and the nucleotide sequence of its promoterGene, 1987
- The mammalian spermatozoonDevelopmental Biology, 1975