Bovine Semen Metabolism. V. Influence of Incubation Temperature on Certain Measures of Fructolysis

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Abstract
Twenty bull semen samples were used to determine the effects of incubation temperature and length of incubation period on fructolysis. Split ejaculates were incubated at 27, 37, and 47[degree]C and fructose utilizations were determined at 60, 120, and 180 minutes. Increase in temperature of incubation resulted in increased utilization, but had no effect on the fructolysis coefficient (Ku). For every 1[degree]C increase in incubation temperature, there was a 4.22% increase in fructose utilization and a 3.84% increase in maximum fructose utilization (MFU). The role of fructose as a rate-limiting factor was suggested by its effect on the 2nd-and 3rd-hour fructose utilizations at 47[degree]C. The routine addition of fructose to all incubated semen samples was suggested, to prevent fructose concentration from becoming a rate-limiting factor.