Isolation and Partial Characterization of Collagen Fibrils, Fibers and Fiber-Bundles From Insoluble Calf Dermis

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Abstract
Collagen fibrils, fibers and fiber bundles have been isolated from insoluble calf skin chips by disaggregation with a neutral salt buffer followed by differential centrifugation. The ratio of type I collagen/type III collagen was relatively constant, regardless of the size of the fibrous materials. On the other hand, the ratios of glyco-saminoglycans/collagen and non-collagenous glycoproteins/collagen were significantly higher in the thinner fiber fractions. However, the ratio of hyaluronic acid to dermatan sulfate was unchanged. The role of the non-collagenous constituents in collagen fibrillogenesis in vivo is discussed.